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KMID : 0900919910150030189
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Korean journal of Animal Reproduction
1991 Volume.15 No. 3 p.189 ~ p.193
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Effect of X-537A on Hydrogen Ion Concentration in Sperm Washed Solution and Sperm Acrosome Reacton in Bovine
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Park Young-Seek
Lim Kyung-Sun
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Abstract
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This study was carried out ot investigate effects of X-537A on hydrogen ion concentration insperm washed solution and sperm acrosome reaction. The results obtained were as follows. 1. When bovine sperm was twice washed with SHP solutions of pH 6.8 and 7.4 and again washed with SHP solution containing 4muM of X-537A, in case of pH 6.8 the sperm washed with 4muM of X-537A showed signifciantly(p<0.01) higher hydrogen ion concentration in sperm washed solution than the sperm washed without X-537A. 2. When the sperm was twice washed with SHP solution and then washed with SHP solution containing 4muM of X-537A, sperm acrosome rection rate was signifciantly(p<0.01) increased from 12min after incubation in the sperm washed without X-537A, but was signifciantly(p<0.01) increased from 8 min after incubation in the sperm washed with 4muM of X-537A. 3. When the sperm was twice washed with SHP solution and then washed with SHP solution containing 0, 4 and 40muM of X-537A, and then incubated in m-TALP for 120 min, sperm acrosome reaction rate was significantly(p<0.01) increased from 15 min after incubation in 0, 4 and 40mum OF X-537A. However at 60 min incubation 40muM of X-537A showed significantly(p<0.01) higher sperm acrosome reaction rate than 0 and 4muM and at 120 min incubation 4 and 40muM of X-537A showed signifciantly(p<0.01) higher acrosome reaction rate than 0muM of X-537A.
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